The KPNA6 Antibody (CAB7363) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, produced through immunization of rabbits, exhibits high specificity and sensitivity towards human samples, making it an excellent choice for Western blot applications. By binding to the KPNA6 protein, this antibody enables precise detection and analysis in various cell types, facilitating in-depth exploration of nucleocytoplasmic transport mechanisms.KPNA6, also known as karyopherin alpha 6, is essential for the transportation of proteins into the nucleus, playing a crucial role in gene expression regulation and cell function.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
KPNA6 Antibody
SKU:
CAB7363
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IPOA7, KPNA6
Positive Sample:
HeLa, Mouse testis
Cellular Localization:
Cytosol, Nucleoplasm, Nucleus.
Calculated MW:
60kDa
Observed MW:
60kDa
Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. The protein encoded by this gene is a member of the importin alpha family.
Purification Method
Affinity purification
Gene ID
23633
RRID
AB_2767897
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using KPNA6 Rabbit pAb (CAB7363) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
