The Cytokeratin 9 (KRT9) Antibody (CAB10119) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blotting. By binding specifically to the KRT9 protein, this antibody enables accurate detection and analysis in different cell types, making it ideal for studies in dermatology and skin biology.Keratin 9 is a structural protein that plays a key role in maintaining the integrity and strength of the skin. Mutations in the KRT9 gene have been associated with various skin disorders, making it a target of interest for researchers studying conditions such as pachyonychia congenita and epidermolytic palmoplantar keratoderma.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Cytokeratin 9 (KRT9) Antibody
SKU:
CAB10119
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes the type I keratin 9, an intermediate filament chain expressed only in the terminally differentiated epidermis of palms and soles. Mutations in this gene cause epidermolytic palmoplantar keratoderma.
Purification Method
Affinity purification
Gene ID
3857
RRID
AB_2757643
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse brain, using Cytokeratin 9 (KRT9) Rabbit pAb (CAB10119) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of paraffin-embedded human skin using Cytokeratin 9 (KRT9) Rabbit pAb (CAB10119) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
