The KYNU Antibody (CAB6643) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and is suitable for use in Western blot applications. By binding to the kynureninase protein, this antibody enables researchers to detect and analyze the enzyme in a variety of cell types, making it a valuable resource for studies in immunology, neuroscience, and metabolic disorders.Kynureninase plays a crucial role in the catabolism of tryptophan, a key amino acid involved in immune regulation and neurotransmitter synthesis. Dysregulation of the kynurenine pathway has been implicated in a variety of diseases, including autoimmune disorders, neurodegenerative diseases, and psychiatric disorders.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
KYNU Antibody
SKU:
CAB6643
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
KYNUU, VCRL2, KYNU
Positive Sample:
A549
Cellular Localization:
Cytoplasm.
Calculated MW:
52kDa
Observed MW:
52kDa
Kynureninase is a pyridoxal-5'-phosphate (pyridoxal-P) dependent enzyme that catalyzes the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively. Kynureninase is involved in the biosynthesis of NAD cofactors from tryptophan through the kynurenine pathway. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
8942
RRID
AB_2767232
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using KYNU Rabbit pAb (CAB6643) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): HAP1 Exposure time: 45s.
Immunohistochemistry analysis of paraffin-embedded Mouse heart using KYNU Rabbit pAb (CAB6643) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
