The L2HGDH Antibody (CAB7996) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific and reacts with human samples, making it suitable for a variety of research applications, particularly in the field of metabolism and metabolic disorders.L2HGDH is an important enzyme that plays a role in maintaining cellular metabolism and energy production. Mutations in the L2HGDH gene have been associated with L-2-hydroxyglutaric aciduria, a rare metabolic disorder characterized by elevated levels of L-2-hydroxyglutaric acid in the urine.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
L2HGDH Antibody
SKU:
CAB7996
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
L2HGA, C14orf160, L2HGDH
Positive Sample:
Mouse kidney, Mouse heart
Cellular Localization:
Mitochondrion.
Calculated MW:
50kDa
Observed MW:
40-50kDa
This gene encodes L-2-hydroxyglutarate dehydrogenase, a FAD-dependent enzyme that oxidizes L-2-hydroxyglutarate to alpha-ketoglutarate in a variety of mammalian tissues. Mutations in this gene cause L-2-hydroxyglutaric aciduria, a rare autosomal recessive neurometabolic disorder resulting in moderate to severe cognitive disability.
Purification Method
Affinity purification
Gene ID
79944
RRID
AB_2770137
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using L2HGDH Rabbit pAb (CAB7996) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Williams et al.
Signalling metabolite L-2-hydroxyglutarate activates the transcription factor HIF-1α in lipopolysaccharide-activated macrophages
