The LAMA2 Antibody (PAC05686) is a polyclonal antibody designed for research involving LAMA2, a protein that plays a key role in the extracellular matrix, particularly in muscle development and stability. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the LAMA2 protein, it allows for detection and analysis in various cell types, making it well-suited for studies in muscular dystrophy, muscle regeneration, and other related fields.
LAMA2, also known as laminin alpha-2, is essential for maintaining muscle integrity and function. Mutations in the LAMA2 gene have been linked to various forms of muscular dystrophy, highlighting the importance of understanding its role in muscle biology. Research into LAMA2 can provide insights into muscle degenerative disorders and potential therapeutic targets for treating these conditions. By studying the function of LAMA2, researchers can uncover new strategies for preventing and treating muscle-related diseases.
IHC image of PACO56868 diluted at 1:200 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of NIH/3T3 cells with PACO56868 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Synonyms:
Laminin subunit alpha-2 (Laminin M chain) (Laminin-12 subunit alpha) (Laminin-2 subunit alpha) (Laminin-4 subunit alpha) (Merosin heavy chain), LAMA2, LAMM
UniProt Protein Function:
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
UniProt Protein Details:
NCBI Summary:
Laminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene. [provided by RefSeq, Jul 2008]
