The LAPTM5 Antibody (CAB17995) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding to the LAPTM5 protein, it enables precise detection and analysis in various cell types, making it an essential component for studies in molecular biology and lysosomal biology.LAPTM5, also known as lysosome-associated protein transmembrane 5, plays a crucial role in maintaining cellular homeostasis by facilitating the degradation of proteins within lysosomes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
LAPTM5 Antibody
SKU:
CAB17995
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CLAST6, LAPTM5
Positive Sample:
HL-60, THP-1, U-937, Mouse spleen, Rat thymus
Cellular Localization:
Cytoplasmic Vesicle, Cytosol, Lysosome, Perinuclear Region Of Cytoplasm, Plasma Membrane, Transport Vesicle.
Calculated MW:
30kDa
Observed MW:
25kDa
This gene encodes a transmembrane receptor that is associated with lysosomes. The encoded protein, also known as E3 protein, may play a role in hematopoiesis.
Purification Method
Affinity purification
Gene ID
7805
RRID
AB_2861791
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using LAPTM5 Rabbit pAb (CAB17995) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of lysates from Rat thymus, using LAPTM5 Rabbit pAb (CAB17995) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.