The LATS1 Monoclonal Antibody (CAB22287) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using hybridoma technology, specifically targets the LATS1 protein and is highly reactive in human samples. It has been validated for use in Western blot, immunofluorescence, and immunohistochemistry applications, allowing for precise detection and analysis of LATS1 in various cell types.LATS1, a member of the Hippo signaling pathway, plays a critical role in controlling cell proliferation, apoptosis, and tissue homeostasis. Dysregulation of LATS1 has been linked to various diseases, including cancer, making it a promising target for therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
LATS1 Monoclonal Antibody
SKU:
CAB22287
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC56891
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.
Purification Method
Affinity purification
Gene ID
9113
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from SH-SY5Y cells, using LATS1 Rabbit mAb (CAB22287) at1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
