The LDHA Monoclonal Antibody (CAB21893) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using monoclonal technology, specifically targets LDHA and is highly reactive with human samples. Validated for use in various applications such as Western blotting and immunohistochemistry, this antibody enables the detection and analysis of LDHA expression in different cell types. Its versatility and specificity make it an ideal choice for studies focused on metabolism, cancer research, and other related fields.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
LDHA Monoclonal Antibody
SKU:
CAB21893
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0509
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
RVHP VSTM IKGL YGI
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:10000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LDHM, GSD11, PIG19, HEL-S-133P, LDHA
Positive Sample:
HeLa, 293T, MCF7, Mouse liver, Rat lung, Rat kidney
Cellular Localization:
Cytoplasm.
Calculated MW:
26-39kDa
Observed MW:
37kDa
The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.
Purification Method
Affinity purification
Gene ID
3939
RRID
AB_3712569
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using LDHA Rabbit mAb (CAB21893) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
