The LDLR Antibody (CAB14996) is a high-quality antibody developed for reliable detection and analysis of target proteins. The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. The encoded protein is normally bound at the cell membrane, where it binds low density lipoprotein/cholesterol and is taken into the cell. Lysosomes release the cholesterol, which is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants. RRID AB_2761879 Gene ID 3949 Swiss Prot Synonym FH; FHC; FHCL1; LDLCQ2; LDL Receptor (LDLR)
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
LDLR Antibody
SKU:
CAB14996
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:50 - 1:200
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FH, FHC, FHCL1, LDLCQ2, LDL Receptor (LDLR)
Positive Sample:
HeLa, HepG2, Mouse lung, Mouse liver, Rat brain
Cellular Localization:
Cell Membrane, Cell Surface, Early Endosome, Endomembrane System, Golgi Apparatus, Late Endosome, Lysosome, Membrane, Single-Pass Type I Membrane Protein, Clathrin-Coated Pit.
Calculated MW:
95kDa
Observed MW:
100-160kDa
The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. The encoded protein is normally bound at the cell membrane, where it binds low density lipoprotein/cholesterol and is taken into the cell. Lysosomes release the cholesterol, which is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants. RRID AB_2761879 Gene ID 3949 Swiss Prot Synonym FH; FHC; FHCL1; LDLCQ2; LDL Receptor (LDLR)
Purification Method:
Affinity purification
Gene ID:
3949
RRID:
AB_2761879
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using LDL Receptor (LDLR) Rabbit pAb (CAB14996) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of lysates from Rat brain, using LDL Receptor (LDLR) Rabbit pAb (CAB14996) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of NIH/3T3 cells using LDL Receptor (LDLR) Rabbit pAb (CAB14996) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
