The LEP Antibody (CAB1300) is a high-quality antibody developed for reliable detection and analysis of target proteins. Leptin, also known as the satiety hormone, plays a crucial role in energy balance by signaling the brain to reduce appetite and increase energy expenditure.Raised in rabbits, this antibody is highly specific and sensitive for detecting leptin in human samples. It has been validated for use in Western blot applications, allowing for the precise detection and quantification of leptin protein levels in various tissues and cell types.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
LEP Antibody
SKU:
CAB1300
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
OB, OBS, LEPD, Leptin
Positive Sample:
HepG2, BxPC-3, RD, Mouse kidney, Rat testis
Cellular Localization:
Secreted.
Calculated MW:
19kDa
Observed MW:
15kDa
This gene encodes a protein that is secreted by white adipocytes into the circulation and plays a major role in the regulation of energy homeostasis. Circulating leptin binds to the leptin receptor in the brain, which activates downstream signaling pathways that inhibit feeding and promote energy expenditure. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis, reproduction, bone formation and wound healing. Mutations in this gene and its regulatory regions cause severe obesity and morbid obesity with hypogonadism in human patients. A mutation in this gene has also been linked to type 2 diabetes mellitus development.
Purification Method
Affinity purification
Gene ID
3952
RRID
AB_2759847
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Leptin Rabbit pAb (CAB1300) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of HepG2 cells using Leptin Rabbit pAb (CAB1300) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
