The LFNG Polyclonal Antibody (CAB7441) is a valuable tool for researchers studying LFNG, a key regulator of the Notch signaling pathway. This antibody, produced in rabbits, exhibits high specificity and reactivity with human samples, making it an ideal choice for various research applications. Validated for use in Western blotting, the LFNG Polyclonal Antibody binds specifically to the LFNG protein, facilitating its detection and analysis in different cell types.LFNG is essential for modulating the activation of Notch signaling, which plays a crucial role in cell development, differentiation, and proliferation.
Dysregulation of this pathway has been implicated in various diseases, including cancer, developmental disorders, and cardiovascular diseases. By targeting LFNG, researchers can gain insight into the mechanisms underlying these conditions and potentially identify novel therapeutic targets for intervention.Overall, the LFNG Polyclonal Antibody is a valuable asset for investigators interested in exploring the role of LFNG in different biological contexts, with the potential to advance our understanding of Notch signaling and its implications for human health.
Product Name:
LFNG Rabbit Polyclonal Antibody
SKU:
CAB7441
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human LFNG (NP_002295.1).
Golgi apparatus membrane, Single-pass type II membrane protein.
Calculated MW:
42kDa
Observed MW:
42kDa
This gene is a member of the glycosyltransferase 31 gene family. Members of this gene family, which also includes the MFNG (GeneID: 4242) and RFNG (GeneID: 5986) genes, encode evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, these proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. The protein encoded by this gene is predicted to be a single-pass type II Golgi membrane protein but it may also be secreted and proteolytically processed like the related proteins in mouse and Drosophila (PMID: 9187150). Mutations in this gene have been associated with autosomal recessive spondylocostal dysostosis 3.
Purification Method:
Affinity purification
Gene ID:
3955
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using LFNG antibody (CAB7441) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 30s.
