The DNA Ligase III/LIG3 Polyclonal Antibody (CAB1887) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody is highly specific and reactive with human samples, making it perfect for use in Western blot applications.Lig3 plays a crucial role in maintaining genomic stability and preventing DNA damage accumulation. Dysregulation of Lig3 function has been linked to various diseases, including cancer and neurodegenerative disorders. By targeting the Lig3 protein, researchers can gain insights into its function and potentially develop therapeutic strategies for conditions related to DNA repair deficiencies.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
DNA Ligase III/LIG3 Polyclonal Antibody
SKU:
CAB1887
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LIG2, MTDPS20, LIG3alpha, DNA Ligase III/LIG3
Positive Sample:
BT474, SW480, HeLa, HT-29, Jurkat
Cellular Localization:
Mitochondrion, Mitochondrion, Nucleus.
Calculated MW:
113kDa
Observed MW:
113kDa
This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Purification Method
Affinity purification
Gene ID
3980
RRID
AB_2763920
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DNA Ligase III/LIG3 Rabbit pAb (CAB1887) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation analysis of 200 μg extracts of Jurkat cells using 1 μg DNA Ligase III/LIG3 antibody (CAB1887). Western blot was performed from the immunoprecipitate using DNA Ligase III/LIG3 antibody (CAB1887) at a dilution of 1:1000.
