The DNA Ligase III/LIG3 Polyclonal Antibody (CAB1887) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
DNA Ligase III/LIG3 Polyclonal Antibody
SKU:
CAB1887
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:5000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LIG2, MTDPS20, LIG3alpha, DNA Ligase III/LIG3
Positive Sample:
BT474, SW480, HeLa, HT-29, Jurkat
Cellular Localization:
Mitochondrion, Mitochondrion, Nucleus.
Calculated MW:
113 kDa
Observed MW:
113 kDa
This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Purification Method
Affinity purification
Gene ID
3980
RRID
AB_2763920
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DNA Ligase III/LIG3 Rabbit pAb (CAB1887) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation analysis of 200 μg extracts of Jurkat cells using 1 μg DNA Ligase III/LIG3 antibody (CAB1887). Western blot was performed from the immunoprecipitate using DNA Ligase III/LIG3 antibody (CAB1887) at a dilution of 1:1000.
