The LIPF Antibody (CAB7016) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and is highly specific for Lipase F in human samples, making it suitable for a variety of research applications, including Western blot analysis. By binding to Lipase F, this antibody enables accurate detection and analysis of the enzyme in different cell types, which is essential for research in areas such as lipid metabolism, obesity, and cardiovascular disease.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
LIPF Antibody
SKU:
CAB7016
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GL, HGL, HLAL, LIPF
Positive Sample:
MCF7, Mouse small intestine, Mouse lung, Mouse heart, Mouse liver, Mouse stomach, Rat kidney
Cellular Localization:
Secreted.
Calculated MW:
45kDa
Observed MW:
45kDa
This gene encodes gastric lipase, an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions. The gene is a member of a conserved gene family of lipases that play distinct roles in neutral lipid metabolism. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
8513
RRID
AB_2767572
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using LIPF Rabbit pAb (CAB7016) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
