The LSM4 Antibody (CAB5891) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of the LSm family of RNA-binding proteins. LSm proteins form stable heteromers that bind specifically to the 3'-terminal oligo(U) tract of U6 snRNA and may play a role in pre-mRNA splicing by mediating U4/U6 snRNP formation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. RRID AB_2766639 Gene ID 25804 Swiss Prot Synonym GRP; YER112W; LSM4
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
LSM4 Antibody
SKU:
CAB5891
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GRP, YER112W, LSM4
Positive Sample:
K-562, 293T, Mouse thymus
Cellular Localization:
Nucleus.
Calculated MW:
15kDa
Observed MW:
16kDa
This gene encodes a member of the LSm family of RNA-binding proteins. LSm proteins form stable heteromers that bind specifically to the 3'-terminal oligo(U) tract of U6 snRNA and may play a role in pre-mRNA splicing by mediating U4/U6 snRNP formation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. RRID AB_2766639 Gene ID 25804 Swiss Prot Synonym GRP; YER112W; LSM4
Purification Method:
Affinity purification
Gene ID:
25804
RRID:
AB_2766639
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using LSM4 Rabbit pAb (CAB5891) at 1:1500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunoprecipitation analysis of 200 μg extracts of Jurkat cells using 1 μg LSM4 antibody (CAB5891). Western blot was performed from the immunoprecipitate using LSM4 antibody (CAB5891) at a dilution of 1:1000.
