The CD68 Antibody (CAB6554) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blotting.MacroSialin is essential for proper lysosomal function and plays a key role in the degradation of glycoproteins within the cell. Dysregulation of MacroSialin has been implicated in lysosomal storage disorders and other related diseases, making it an important target for further investigation.
This antibody is validated for use in - applications and has demonstrated reactivity against - samples.
Western blot analysis of various lysates using CD68 Rabbit pAb (CAB6554) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of THP-1 cells using CD68 Rabbit pAb (CAB6554) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of RAW264.7 cells using CD68 Rabbit pAb (CAB6554) at dilution of 1:250. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
