The MAD2/MAD2L1 Monoclonal Antibody (CAB11469) is a high-quality antibody developed for reliable detection and analysis of target proteins. MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. RRID AB_2861572 Gene ID 4085 Swiss Prot Synonym MAD2; HSMAD2; MAD2/MAD2L1
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
MAD2/MAD2L1 Monoclonal Antibody
SKU:
CAB11469
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC0603
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MAD2, HSMAD2, MAD2/MAD2L1
Positive Sample:
HT-29, HepG2, Raji
Cellular Localization:
Chromosome, Cytoplasm, Nucleus, Centromere, Cytoskeleton, Kinetochore, Spindle Pole.
Calculated MW:
24kDa
Observed MW:
24kDa
MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. RRID AB_2861572 Gene ID 4085 Swiss Prot Synonym MAD2; HSMAD2; MAD2/MAD2L1
Purification Method:
Affinity purification
Gene ID:
4085
RRID:
AB_2861572
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MAD2/MAD2/MAD2L1 Rabbit mAb (CAB11469) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
