The MAK16 Antibody (CAB20182) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and exhibits high reactivity with human samples, making it suitable for use in Western blot applications. By binding specifically to the MAK16 protein, this antibody enables accurate detection and analysis in various cell types, making it an ideal choice for studies in molecular biology and genetic research.MAK16 is known for its crucial role in maintaining genomic stability and ensuring proper chromosome segregation during cell division.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MAK16 Antibody
SKU:
CAB20182
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RBM13, MAK16L, MAK16
Positive Sample:
Mouse liver, Mouse testis, Rat testis
Cellular Localization:
Nucleolus.
Calculated MW:
35kDa
Observed MW:
34kDa
Enables RNA binding activity. Predicted to be involved in maturation of 5.8S rRNA and maturation of LSU-rRNA. Located in nucleolus.
Purification Method
Affinity purification
Gene ID
84549
RRID
AB_2862969
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MAK16 Rabbit pAb (CAB20182) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of lysates from Rat testis, using MAK16 Rabbit pAb (CAB20182) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
