The MAN1A2 Antibody (CAB14271) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, produced in rabbits, shows high reactivity with human samples and is validated for use in various applications such as Western blot and immunohistochemistry.MAN1A2, a key enzyme in the glycosylation pathway, plays a crucial role in the quality control of glycoprotein folding and assembly in the endoplasmic reticulum. Dysregulation of MAN1A2 has been implicated in various diseases, making it a target of interest for researchers studying protein glycosylation disorders, cancer, and neurodegenerative diseases.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
MAN1A2 Antibody
SKU:
CAB14271
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MAN1B, MAN1A2
Positive Sample:
LO2, A-549, Mouse brain, Mouse liver
Cellular Localization:
Golgi Apparatus Membrane, Single-Pass Type Ii Membrane Protein.
Calculated MW:
73kDa
Observed MW:
73kDa
Alpha-mannosidases function at different stages of N-glycan maturation in mammalian cells. See MAN2A1 (MIM 154582) for general information.
Purification Method
Affinity purification
Gene ID
10905
RRID
AB_2761134
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MAN1A2 Rabbit pAb (CAB14271) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using MAN1A2 Rabbit pAb (CAB14271) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
