The MANEA Polyclonal Antibody (CAB10756) is a valuable tool for researchers studying MANEA, a key enzyme involved in the biosynthesis of N-glycans. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been specifically validated for use in various applications, including Western blotting.MANEA, also known as Mannosidase Endo-α, plays a crucial role in the processing of N-glycans, which are essential for protein folding, stability, and function. Dysregulation of N-glycan biosynthesis has been linked to various diseases, including cancer and genetic disorders.
By targeting MANEA, researchers can gain insight into the mechanisms underlying these pathologies and explore potential therapeutic interventions.With its ability to specifically detect and bind to MANEA, this polyclonal antibody provides researchers with a powerful tool for investigating the role of N-glycans in health and disease. Its utility in a range of experimental settings makes it an essential component of any immunology or glycoscience research program.
Product Name:
MANEA Rabbit Polyclonal Antibody
SKU:
CAB10756
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-100 of human MANEA (NP_078917.2).
Golgi apparatus membrane, Single-pass type II membrane protein.
Calculated MW:
54kDa
Observed MW:
54kDa
N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 [PubMed 15677381]).
Purification Method:
Affinity purification
Gene ID:
79694
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MANEA Rabbit pAb (CAB10756) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
