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Anti-LC3B Antibody (CAB5601)

SKU:
CAB5601
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Autophagy
  • Autophagy Antibodies Anti-LC3B Antibody CAB5601
  • Autophagy Antibodies Anti-LC3B Antibody CAB5601
  • Autophagy Antibodies Anti-LC3B Antibody CAB5601
  • Autophagy Antibodies Anti-LC3B Antibody CAB5601
  • Autophagy Antibodies Anti-LC3B Antibody CAB5601
€139 - €419
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Description

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Antibody Name:Anti-LC3B Antibody
Antibody SKU:CAB5601
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LC3B (NP_073729.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, NIH/3T3, C6, Mouse heart, Mouse brain, Rat heart
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LC3B (NP_073729.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPSE KTFK QRRT FEQR VEDV RLIR EQHP TKIP VIIE RYKG EKQL PVLD KTKF LVPD HVNM SELI KIIR RRLQ LNAN QAFF LLVN GHSM VSVS TPIS EVYE
Gene ID:81631
Uniprot:Q9GZQ8
Cellular Location:Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton
Calculated MW:14kDa
Observed MW:14kDa/16kDa
Synonyms:LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, MAP1LC3B, LC3A/LC3B
Background: The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
NCBI Summary:The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]
UniProt Code:Q9GZQ8
NCBI GenInfo Identifier:17433141
NCBI Gene ID:81631
NCBI Accession:Q9GZQ8.3
UniProt Related Accession:Q9GZQ8
Molecular Weight:
NCBI Full Name:Microtubule-associated proteins 1A/1B light chain 3B
NCBI Synonym Full Names:microtubule associated protein 1 light chain 3 beta
NCBI Official Symbol:MAP1LC3B  
NCBI Official Synonym Symbols:LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3  
NCBI Protein Information:microtubule-associated proteins 1A/1B light chain 3B
UniProt Protein Name:Microtubule-associated proteins 1A/1B light chain 3B
UniProt Synonym Protein Names:Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta
UniProt Gene Name:MAP1LC3B  
UniProt Entry Name:MLP3B_HUMAN
Anti-LC3B Antibody (CAB5601)Western blot analysis of extracts of NIH/3T3 cells, using LC3B antibody (CAB5601) at 1:1000 dilution. NIH/3T3 cells were treated by Chloroquine (50 uM) for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
Anti-LC3B Antibody (CAB5601)Western blot analysis of extracts of various cell lines, using LC3B antibody (CAB5601) at 1:1000 dilution.Hela cells were treated by Chloroquine (50 uM) at 37'C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-LC3B Antibody (CAB5601)Immunohistochemistry of paraffin-embedded rat brain using LC3B Rabbit pAb (CAB5601) at dilution of 1:100 (40x lens).
Anti-LC3B Antibody (CAB5601)Immunohistochemistry of paraffin-embedded human colon using LC3B Rabbit pAb (CAB5601) at dilution of 1:100 (40x lens).
Anti-LC3B Antibody (CAB5601)Immunohistochemistry of paraffin-embedded mouse brain using LC3B Rabbit pAb (CAB5601) at dilution of 1:100 (40x lens).

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