The MEK1 Antibody (CAB0252) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. RRID AB_2757065 Gene ID 5604 Swiss Prot Synonym MEL; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MEK1 Antibody
SKU:
CAB0252
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF
/
ICC
1:50 - 1:100
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MEL, CFC3, MEK1, MKK1, MAPKK1, PRKMK1
Positive Sample:
Mouse lung, Mouse stomach, Mouse brain
Cellular Localization:
Cytoplasm, Membrane, Nucleus, Peripheral Membrane Protein, Centrosome, Cytoskeleton, Microtubule Organizing Center, Spindle Pole Body.
Calculated MW:
43kDa
Observed MW:
46kDa
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. RRID AB_2757065 Gene ID 5604 Swiss Prot Synonym MEL; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
Purification Method:
Affinity purification
Gene ID:
5604
RRID:
AB_2757065
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MEK1 Rabbit pAb (CAB0252). Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of U2OS cells using MEK1 Rabbit pAb (CAB0252). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
