The MAP2K5 Antibody (PAC021465) is a polyclonal antibody designed for research involving the MAP2K5 protein, which is a key player in the MAPK signaling pathway. This pathway is involved in cell growth, differentiation, and response to environmental stressors. Raised in rabbits, this antibody is highly reactive with human samples and has been validated for use in Western blot applications. It binds specifically to the MAP2K5 protein, allowing for accurate detection and analysis in a variety of cell types.
This makes it an ideal tool for researchers studying cell signaling, cancer biology, and drug development.The MAP2K5 protein is known to regulate cell proliferation, survival, and migration, making it a potential target for therapeutic interventions in diseases like cancer and inflammatory conditions. Understanding the role of MAP2K5 in cellular signaling pathways is essential for developing targeted therapies and advancing our understanding of disease mechanisms.
Antibody Name:
Map2k5 Antibody (PACO21465)
Antibody SKU:
PACO21465
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:1000
Species Reactivity:
Human, Rat, Mouse
Immunogen:
Peptide sequence around aa. 311-315(K-T-Y-V-G ) derived from Rat MEK5.
Form:
Liquid
Storage Buffer:
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extract from rat brain and mouse brain tissue using MEK5 Antibody.
Background:
A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phospho-rylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resem-bles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphory-late ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7)phosphorylates and activates the JNK signal transduction pathway. Jiang, Y., et al. 1996. J. Biol. Chem. 271: 17920-17926. Holland, P.M., et al. 1997. J. Biol. Chem. 272: 24994-24998.
MEK5: a dual specificity protein kinase of the STE7 family. Activates ERK5 and is itself phosphorylated and activated by MEKK3, as well as by atypical protein kinase C isoforms. Involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced isoforms have been described.Protein type: EC 2.7.12.2; Kinase, protein; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); STE group; STE7 familyCellular Component: membrane; spindle; intracellular; nucleus; cytosolMolecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; metal ion binding; protein-tyrosine kinase activity; ATP binding; protein kinase activityBiological Process: peptidyl-tyrosine phosphorylation; negative regulation of heterotypic cell-cell adhesion; activation of MAPK activity; heart development; MAPKKK cascade; negative regulation of interleukin-8 biosynthetic process; negative regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; positive regulation of protein metabolic process; positive regulation of cell growth; inhibition of NF-kappaB transcription factor; positive regulation of transcription from RNA polymerase II promoter; positive regulation of epithelial cell proliferation
UniProt Protein Details:
NCBI Summary:
kinase involved in signal transduction in the cell [RGD, Feb 2006]
