The MAP3K14 Antibody (CAB11585) is a high-quality antibody developed for reliable detection and analysis of target proteins. NIK is a key regulator of the NF-κB pathway, playing a crucial role in immune and inflammatory responses.This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By specifically binding to the MAP3K14 protein, researchers can accurately detect and analyze its expression in various cell types.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
Product Name:
MAP3K14 Antibody
SKU:
CAB11585
Size:
20μL, 100μL
Reactivity:
Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HS, NIK, HSNIK, FTDCR1B, MAP3K14
Positive Sample:
Rat lung
Cellular Localization:
Cytoplasm.
Calculated MW:
104kDa
Observed MW:
125kDa
This gene encodes mitogen-activated protein kinase kinase kinase 14, which is a serine/threonine protein-kinase. This kinase binds to TRAF2 and stimulates NF-kappaB activity. It shares sequence similarity with several other MAPKK kinases. It participates in an NF-kappaB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family and to the interleukin-1 type-I receptor.
Purification Method
Affinity purification
Gene ID
9020
RRID
AB_2758614
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat lung, using MAP3K14 Rabbit pAb (CAB11585) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
