Description
MAP3K4 Antibody (CAB16918)
The MAP3K4 Antibody (CAB16918) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody is highly specific to human MAP3K4 and has been validated for use in Western blot applications. By binding to the MAP3K4 protein, this antibody allows for accurate detection and analysis of MAP3K4 expression in various cell types.MAP3K4, also known as mitogen-activated protein kinase kinase kinase 4, plays a crucial role in regulating cell proliferation, apoptosis, and response to environmental stressors.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Rat samples.
| Product Name: | MAP3K4 Antibody |
| SKU: | CAB16918 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Rat |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||||
| Sequence: | MREA AAAL VPPP AFAV TPAA AMEE PPPP PPPP PPPP EPET ESEP ECCL AARQ EGTL GDSA CKSP ESDL EDFS DETN TENL YGTS PPST PRQM KRMS TKHQ | ||||||
| Tested Applications: | WB IHC-P ELISA | ||||||
| Recommended Dilution: |
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| Synonyms: | MTK1, MEKK4, MEKK 4, MAPKKK4, PRO0412, MAP3K4 |
| Positive Sample: | K-562, Rat brain |
| Cellular Localization: | Cytoplasm, Perinuclear Region Of Cytoplasm. |
| Calculated MW: | 182kDa |
| Observed MW: | 170kDa |
The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Several alternatively spliced transcripts encoding distinct isoforms have been described.
| Purification Method | Affinity purification |
| Gene ID | 4216 |
| RRID | AB_2770267 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using MAP3K4 Rabbit pAb (CAB16918) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s. |
 | Immunohistochemistry analysis of paraffin-embedded Human lung cancer using MAP3K4 Rabbit pAb (CAB16918) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human placenta using MAP3K4 Rabbit pAb (CAB16918) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
