The MAP3K5 Antibody (CAB12458) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high specificity for human samples and has been validated for use in Western blot applications. By targeting the MAP3K5 protein, this antibody allows for precise detection and analysis in various cell types, making it ideal for studies in immunology, cancer research, and other fields where MAP3K5 is implicated.MAP3K5, also known as apoptosis signal-regulating kinase 1 (ASK1), plays a crucial role in regulating cell death and survival pathways, making it a promising target for research into diseases such as cancer, neurodegenerative disorders, and cardiovascular diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MAP3K5 Antibody
SKU:
CAB12458
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ASK1, MEKK5, MAPKKK5, MAP3K5
Positive Sample:
HeLa, A-549, BxPC-3, A-431, Mouse lung, Mouse brain, Mouse heart, Rat lung, Rat brain
Cellular Localization:
Cytoplasm, Endoplasmic Reticulum.
Calculated MW:
155kDa
Observed MW:
160kDa
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
Purification Method
Affinity purification
Gene ID
4217
RRID
AB_2759301
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using MAP3K5 Rabbit pAb (CAB12458) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
