The MAPT Polyclonal Antibody (CAB23656) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been rigorously validated for use in Western blot applications. By binding specifically to the MAPT protein, researchers can accurately detect and analyze its expression in a variety of cell types, making it an essential tool for investigations in neurobiology and neurodegenerative disease research.MAPT, also known as microtubule-associated protein tau, plays a vital role in the stabilization and assembly of microtubules in neurons.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MAPT Polyclonal Antibody
SKU:
CAB23656
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:1000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Enables DNA binding activity; microtubule binding activity; and protein kinase binding activity. Involved in negative regulation of tubulin deacetylation; regulation of cellular response to heat; and regulation of response to DNA damage stimulus. Acts upstream of or within several processes, including adult walking behavior; generation of neurons; and transport along microtubule. Located in several cellular components, including cytoskeleton; glial cell projection; and postsynaptic density. Is expressed in several structures, including gut; nervous system; sensory organ; smooth muscle tissue; and urinary system. Used to study Alzheimer's disease. Human ortholog(s) of this gene implicated in dementia (multiple); neurodegenerative disease (multiple); progressive supranuclear palsy; and temporal lobe epilepsy. Orthologous to human MAPT (microtubule associated protein tau).
Purification Method
Affinity purification
Gene ID
17762
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using MAPT Rabbit pAb (CAB23656) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of MCF7 cells using MAPT Rabbit pAb(CAB23656) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
