The March1 Antibody (PACO4671) is a valuable tool for researchers studying the function of March1, a crucial regulator of immune responses. This polyclonal antibody, generated in rabbits, exhibits high specificity and sensitivity towards March1 in human samples, making it ideal for use in Western blotting experiments. By binding specifically to the March1 protein, researchers can confidently detect and analyze its expression in various cell types, providing insights into its role in immune regulation.March1, also known as membrane-associated RING-CH 1, plays a key role in modulating immune signaling pathways by regulating the turnover of cell surface proteins involved in immune responses.
Dysregulation of March1 has been implicated in a variety of autoimmune diseases and inflammatory conditions, making it a promising target for therapeutic interventions. By studying the function of March1, researchers can further our understanding of immune dysregulation and potentially uncover new treatment strategies for immune-related disorders.
Antibody Name:
MARCH1 Antibody
Antibody SKU:
PACO04671
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
IHC:1:100-1:300
Species Reactivity:
Human
Immunogen:
synthesized peptide derived from the Internal region of human 40603.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Synonyms:
MARCH1; RNF171; E3 ubiquitin-protein ligase MARCH1; Membrane-associated RING finger protein 1; Membrane-associated RING-CH protein I; MARCH-I; RING finger protein 171
UniProt Protein Function:
MARCH1: E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, down-regulates their cell surface localization thus sequestering them in the intracellular endosomal system. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Ligase; Ubiquitin conjugating system; EC 6.3.2.19; Membrane protein, multi-pass; EC 6.3.2.-; Membrane protein, integral; Ubiquitin ligaseChromosomal Location of Human Ortholog: 4q32.2Cellular Component: early endosome membrane; endoplasmic reticulum membrane; endosome; late endosome membrane; lysosomal membrane; lysosome; plasma membrane; trans-Golgi network membraneMolecular Function: MHC protein binding; ubiquitin-protein ligase activityBiological Process: antigen processing and presentation of peptide antigen via MHC class II; immune response; protein polyubiquitination
UniProt Protein Details:
NCBI Summary:
MARCH1 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH1 downregulates the surface expression of major histocompatibility complex (MHC) class II molecules (see MIM 142880) and other glycoproteins by directing them to the late endosomal/lysosomal compartment (Bartee et al., 2004 [PubMed 14722266]; Thibodeau et al., 2008 [PubMed 18389477]; De Gassart et al., 2008 [PubMed 18305173]).[supplied by OMIM, Mar 2010]