The March4 Polyclonal Antibody (PACO22319) is a highly specific research tool designed for studies involving the March4 protein. This antibody, generated in rabbits, exhibits strong reactivity with human samples and is validated for use in Western blot applications. By targeting the March4 protein, this antibody enables precise detection and analysis in a variety of cell types, making it a valuable asset for research in stem cell biology, developmental biology, and cancer research.March4, also known as Tumor Initiating Cells-Specific Dna-J Domain-Containing Protein, is a key player in stem cell maintenance and differentiation. It is implicated in various cellular processes, including self-renewal and pluripotency, making it a promising target for investigating stem cell biology and regenerative medicine.
Research into the functions and regulatory mechanisms of March4 is essential for advancing our understanding of stem cell biology and its therapeutic applications in regenerative medicine.Overall, the March4 Polyclonal Antibody (PACO22319) offers researchers a reliable tool for studying the role of the March4 protein in various cellular processes and diseases, providing valuable insights into stem cell biology and potential therapeutic strategies for regenerative medicine.
Antibody Name:
MARCH4 Antibody (PACO22319)
Antibody SKU:
PACO22319
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:50-1:100
Species Reactivity:
Human, Mouse
Immunogen:
Synthesized peptide derived from internal of human MARCH4.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Background:
E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Nagase T., DNA Res. 7:65-73(2000). The MGC Project Team; Genome Res. 14:2121-2127(2004). Bartee E., J. Virol. 78:1109-1120(2004).
Synonyms:
E3 ubiquitin-protein ligase MARCH4 ; EC 6.3.2.-; Membrane-associated RING finger protein 4; Membrane-associated RING-CH protein IV; MARCH-IV
UniProt Protein Function:
MARCH4: E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.Protein type: EC 6.3.2.19; EC 6.3.2.-; Membrane protein, multi-pass; Membrane protein, integral; Ubiquitin ligase; Ligase; Ubiquitin conjugating systemChromosomal Location of Human Ortholog: 2q35Cellular Component: Golgi stack; trans-Golgi networkMolecular Function: ubiquitin-protein ligase activity
UniProt Protein Details:
NCBI Summary:
MARCH4 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH4 reduces surface accumulation of several membrane glycoproteins by directing them to the endosomal compartment (Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Apr 2010]
