The MBD4 Polyclonal Antibody (PACO10407) is a valuable tool for researchers studying MBD4, a protein involved in DNA repair and epigenetic regulation. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, and has been validated for use in Western blot applications.MBD4, also known as Methyl-CpG-binding domain protein 4, plays a crucial role in maintaining genomic stability by recognizing and repairing mismatched bases in DNA. Dysregulation of MBD4 has been implicated in various cancers and other diseases, highlighting its importance in cellular processes.
Researchers in the fields of genomics, cancer biology, and epigenetics can benefit from using the MBD4 Polyclonal Antibody to investigate the function and regulation of MBD4 in different experimental settings. By enabling the detection and analysis of MBD4 protein levels, this antibody offers valuable insights into DNA repair mechanisms and epigenetic modifications, ultimately advancing our understanding of disease pathogenesis and potential therapeutic targets.
MBD4: Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein. 3 isoforms of the human protein are produced by alternative splicing.Protein type: DNA repair, damage; EC 3.2.2.-; Apoptosis; DeoxyribonucleaseChromosomal Location of Human Ortholog: 3q21.3Cellular Component: nucleoplasm; cytoplasm; nucleus; chromatinMolecular Function: protein binding; satellite DNA binding; pyrimidine-specific mismatch base pair DNA N-glycosylase activity; endodeoxyribonuclease activityBiological Process: response to radiation; base-excision repair, AP site formation; DNA damage response, signal transduction resulting in induction of apoptosis; depyrimidination; base-excision repair; mitotic cell cycle G2/M transition DNA damage checkpoint; DNA repair; DNA catabolic process, endonucleolytic
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains an MBD domain at the N-terminus that functions both in binding to methylated DNA and in protein interactions and a C-terminal mismatch-specific glycosylase domain that is involved in DNA repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]
