The MBNL3 Polyclonal Antibody (PACO59389) is a crucial tool for researchers studying MBNL3, a protein involved in RNA splicing and regulation of gene expression. This antibody, manufactured in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blotting. By targeting the MBNL3 protein, this antibody enables precise detection and analysis in a range of cell types, making it an essential component for investigations in molecular biology and disease research.MBNL3, also known as muscleblind-like protein 3, is a key player in RNA metabolism and splicing processes, contributing to the regulation of gene expression and cellular function.
Dysregulation of MBNL3 has been associated with various diseases, including myotonic dystrophy and cancer, underscoring its significance in cellular homeostasis and disease development. By delving into the functions of MBNL3, researchers can uncover novel insights into disease mechanisms and potential therapeutic targets for conditions linked to MBNL3 dysfunction.
IHC image of PACO59389 diluted at 1:400 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with PACO59389 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. May play a role in myotonic dystrophy pathophysiology (DM). Could inhibit terminal muscle differentiation, acting at approximately the time of myogenin induction.
MBNL3: Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. May play a role in myotonic dystrophy pathophysiology (DM). Could inhibit terminal muscle differentiation, acting at approximately the time of myogenin induction. Belongs to the muscleblind family. 5 isoforms of the human protein are produced by alternative splicing.Protein type: RNA-bindingChromosomal Location of Human Ortholog: Xq26.2Molecular Function: protein bindingBiological Process: regulation of RNA splicing
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the muscleblind-like family of proteins. The encoded protein may function in regulation of alternative splicing and may play a role in the pathophysiology of myotonic dystrophy. Alternatively spliced transcript variants have been described. [provided by RefSeq, Dec 2009]
