The MCF2 Antibody (CAB12839) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and exhibits high reactivity with human samples, making it suitable for use in Western blot applications. By binding specifically to the MCF2 protein, this antibody enables researchers to detect and analyze MCF2 expression in various cell types, providing insights into its role in cellular processes.MCF2, also known as guanine nucleotide exchange factor, plays a crucial role in the regulation of cell growth, proliferation, and migration.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
MCF2 Antibody
SKU:
CAB12839
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DBL, ARHGEF21, MCF2
Positive Sample:
HT-29
Cellular Localization:
Cytoplasm, Membrane.
Calculated MW:
108kDa
Observed MW:
108kDa
The oncogenic protein encoded by this gene is a guanine nucleotide exchange factor (GEF) that exerts control over some members of the Rho family of small GTPases. Several transcript variants encoding different isoforms have been found for this gene. These isoforms exhibit different expression patterns and varying levels of GEF activity.
Purification Method
Affinity purification
Gene ID
4168
RRID
AB_2759679
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HT-29 cells, using MCF2 Rabbit pAb (CAB12839) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
