The MCM3 Antibody (CAB1060) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is specifically validated for use in Western blot applications. By targeting the MCM3 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential asset for studies in molecular biology and cancer research.MCM3 is essential for DNA replication and is a marker of cellular proliferation, making it a crucial player in maintaining genomic stability and preventing abnormalities in cell division.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MCM3 Antibody
SKU:
CAB1060
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HCC5, P1.h, RLFB, P1-MCM3, MCM3
Positive Sample:
HeLa, 293T, PC-12, NIH/3T3, COS-1, COS-7
Cellular Localization:
Nucleus.
Calculated MW:
91kDa
Observed MW:
105kDa
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
4172
RRID
AB_2758140
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MCM3 Rabbit pAb (CAB1060) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation analysis of 300 μg extracts of NIH/3T3 cells using 3 μg MCM3 antibody (CAB1060). Western blot was performed from the immunoprecipitate using MCM3 antibody (CAB1060) at a dilution of 1:1000.
