The MCOLN2 Antibody (CAB18560) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody is highly specific to human samples and has been validated for use in various laboratory techniques, including Western blotting.MCOLN2, also known as TRPML2, is a member of the transient receptor potential (TRP) superfamily and plays a crucial role in endolysosomal trafficking and function. Dysregulation of MCOLN2 has been implicated in lysosomal storage disorders and neurodegenerative diseases, making it a promising target for therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
MCOLN2 Antibody
SKU:
CAB18560
Size:
20μL, 100μL
Reactivity:
Human, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TRPML2, TRP-ML2, MCOLN2
Positive Sample:
THP-1, SKOV3
Cellular Localization:
Lysosome, Plasma Membrane.
Calculated MW:
66kDa
Observed MW:
75kDa
Mucolipins constitute a family of cation channel proteins with homology to the transient receptor potential superfamily. In mammals, the mucolipin family includes 3 members, MCOLN1 (MIM 605248), MCOLN2, and MCOLN3 (MIM 607400), that exhibit a common 6-membrane-spanning topology. Homologs of mammalian mucolipins exist in Drosophila and C. elegans. Mutations in the human MCOLN1 gene cause mucolipodosis IV (MIM 262650) (Karacsonyi et al., 2007 [PubMed 17662026]).
Purification Method
Affinity purification
Gene ID
255231
RRID
AB_2862324
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MCOLN2 Rabbit pAb (CAB18560) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
