The MCU Polyclonal Antibody (CAB21753) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for MCU and is validated for use in multiple applications such as Western blot and immunofluorescence.The MCU protein is essential for maintaining calcium homeostasis in mitochondria, which is crucial for various cellular processes including energy production and cell death regulation. Dysregulation of MCU has been linked to various diseases including neurodegenerative disorders and cardiovascular diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MCU Polyclonal Antibody
SKU:
CAB21753
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HsMCU, C10orf42, CCDC109A, MCU
Positive Sample:
Mouse spleen
Cellular Localization:
Mitochondrial Inner Membrane, Mitochondrion.
Calculated MW:
40kDa
Observed MW:
35kDa
Enables calcium channel activity; identical protein binding activity; and uniporter activity. Involved in several processes, including positive regulation of mitochondrial calcium ion concentration; positive regulation of mitochondrial fission; and positive regulation of neutrophil chemotaxis. Acts upstream of or within calcium import into the mitochondrion. Located in mitochondrial inner membrane. Is integral component of mitochondrial inner membrane. Part of uniplex complex.
Purification Method
Affinity purification
Gene ID
90550
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse spleen, using MCU Rabbit pAb (CAB21753) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
