The MED18 Antibody (CAB14588) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and exhibits high reactivity with human samples, making it suitable for use in Western blot applications.MED18 is essential for the activation of gene transcription and plays a crucial role in various cellular processes, including development, differentiation, and cell cycle control. By targeting the MED18 protein, researchers can gain valuable insights into its function and mechanism of action, making this antibody a valuable asset for studies in molecular biology and gene expression regulation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
MED18 Antibody
SKU:
CAB14588
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SRB5, p28b, MED18
Positive Sample:
HT-29, Jurkat, Mouse liver, Mouse thymus
Cellular Localization:
Nucleus.
Calculated MW:
24kDa
Observed MW:
24kDa
MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II (Sato et al., 2003 [PubMed 12584197]).
Purification Method
Affinity purification
Gene ID
54797
RRID
AB_2761462
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MED18 Rabbit pAb (CAB14588) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
