The MED8 Antibody (PACO22405) is a polyclonal antibody specifically designed for research involving MED8, an essential component of the Mediator complex that regulates gene expression. This antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in various applications, including Western blotting.MED8 is a crucial mediator of transcriptional regulation, playing a key role in controlling the expression of genes involved in key cellular processes.
Research on MED8 has implications in understanding various diseases, including cancer, where dysregulation of gene expression can lead to uncontrolled cell growth.By targeting MED8, researchers can gain valuable insights into the molecular mechanisms underlying gene regulation, providing a deeper understanding of cellular processes and potential therapeutic targets. The MED8 Antibody is a valuable tool for studies in molecular biology, cancer research, and beyond.
Antibody Name:
MED8 Antibody (PACO22405)
Antibody SKU:
PACO22405
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthesized peptide derived from Internal of human MED8.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from Jurkat/K562 cells, using MED8 antibody.
Background:
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins.
Synonyms:
Activator-recruited cofactor 32 kDa component; ARC32; MED8; Mediator complex subunit 8; Mediator of RNA polymerase II transcription subunit 8
UniProt Protein Function:
MED8: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins. Belongs to the Mediator complex subunit 8 family. 3 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 1p34.2Cellular Component: nucleoplasm; Srb-mediator complexMolecular Function: protein bindingBiological Process: transcription initiation from RNA polymerase II promoter
UniProt Protein Details:
NCBI Summary:
This gene encodes a protein component of the mediator complex, which aids in transcriptional activation through interaction with RNA polymerase II and gene-specific transcription factors. The encoded protein may also function in ubiquitin ligation and protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]
