MEF2A Transcription Factor Activity Assay



ELISA Kit Technical Manual

MEF2A Transcription Factor activity assay

Product Description:The Assay Genie MEF2A transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates Assay Genie ELISA kits are designed to significantly reduce experiment Time: and ensure sensitivity and flexibility for high-throughput screening.
Target Synonyms:Serum response factor-like protein 1
Detection Target:Phospho-IRAK1(Thr209)
Reactivity:Human, Mouse, Rat
Pack Size:12 x 8-Well Microstrips
Detection Method:Colorimetric 450 nm
Format:transcription factor activity assay
Storage and Stability Guarantee:4 °C/6 Months
Sample Type:Nuclear or cell lysates
Range:See Technical Manual
Time:4.5 hours

MEF2A Transcription Factor activity assay Protein Information

UniProt Protein Function:MEF2A: a myocyte-specific enhancing transcription factor which binds specifically to the MEF2 element present in the regulatory regions of many, if not all, muscle-specific genes. A member of the MADS gene family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain. Five alternatively spliced isoforms have been reported.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 15q26

Cellular Component: cytoplasm; nuclear chromatin; nucleoplasm; nucleus; transcription factor complex

Molecular Function:chromatin binding; histone acetyltransferase binding; histone deacetylase binding; protein binding; protein heterodimerization activity; protein kinase binding; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; SMAD binding; transcription activator binding; transcription factor activity

Biological Process: apoptosis; dendrite morphogenesis; heart development; innate immune response; MAPKKK cascade; mitochondrial genome maintenance; mitochondrion distribution; muscle cell differentiation; muscle development; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; nerve growth factor receptor signaling pathway; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; stress-activated MAPK cascade; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; transcription from RNA polymerase II promoter; transcription, DNA-dependent; ventricular cardiac myofibril development

Disease: Coronary Artery Disease, Autosomal Dominant, 1

NCBI Summary:The protein encoded by this gene is a DNA-binding transcription factor that activates many muscle-specific, growth factor-induced, and stress-induced genes. The encoded protein can act as a homodimer or as a heterodimer and is involved in several cellular processes, including muscle development, neuronal differentiation, cell growth control, and apoptosis. Defects in this gene could be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010]
UniProt Code:Q02078
NCBI GenInfo Identifier:1170908
NCBI Gene ID:4205
NCBI Accession:Q02078.1
UniProt Secondary Accession:Q02078,O43814, Q14223, Q14224, Q59GX4, Q7Z6C9, Q96D14 B4DFQ7, F6XG23,
UniProt Related Accession:Q02078
Molecular Weight:45,570 Da
NCBI Full Name:Myocyte-specific enhancer factor 2A
NCBI Synonym Full Names:myocyte enhancer factor 2A
NCBI Official Symbol:MEF2A
NCBI Official Synonym Symbols:mef2; ADCAD1; RSRFC4; RSRFC9
NCBI Protein Information:myocyte-specific enhancer factor 2A
UniProt Protein Name:Myocyte-specific enhancer factor 2A
UniProt Synonym Protein Names:Serum response factor-like protein 1
Protein Family:Myocyte-specific enhancer factor
UniProt Gene Name:MEF2A
UniProt Entry Name:MEF2A_HUMAN
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Additional Information

Product Type:
Transcription Factor Assay
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