The MEGF9 Polyclonal Antibody (PAC057488) is designed for research involving MEGF9, a cell surface protein involved in various cellular processes, including cell adhesion and phagocytosis. The antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in applications such as Western blotting. By targeting the MEGF9 protein, this antibody allows for the detection and analysis of MEGF9 in different cell types, making it suitable for studies in cell biology and disease research.
MEGF9, also known as Multiple EGF-like domains protein 9, plays a crucial role in regulating cell-cell interactions and phagocytic processes, making it a key player in the immune response and tissue development. Its involvement in these cellular functions makes it a promising target for research in areas such as immunology, cancer biology, and developmental biology. Understanding the function of MEGF9 can provide valuable insights into disease mechanisms and potential therapeutic strategies targeting this protein.
Western Blot. Positive WB detected in: A549 whole cell lysate, PC-3 whole cell lysate. All lanes: MEGF9 antibody at 11.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 63 kDa. Observed band size: 63 kDa.
Immunofluorescence staining of A549 cells with PACO57488 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO57488 diluted at 1:300 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Synonyms:
Multiple epidermal growth factor-like domains protein 9 (Multiple EGF-like domains protein 9) (Epidermal growth factor-like protein 5) (EGF-like protein 5), MEGF9, EGFL5 KIAA0818
UniProt Protein Function:
MEGF9: Protein type: Membrane protein, integralChromosomal Location of Human Ortholog: 9q32-q33.3
