The METAP2 Antibody (CAB5962) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly sensitive and specific for detecting MetaP2 in human samples, making it ideal for use in Western blot applications.MetaP2 plays a crucial role in lipid droplet formation and breakdown, as well as in regulating energy balance in cells. By targeting MetaP2 with this antibody, researchers can gain insights into how this protein contributes to metabolic processes and potentially uncover new therapeutic targets for metabolic disorders like obesity and diabetes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
METAP2 Antibody
SKU:
CAB5962
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MAP2, MNPEP, p67eIF2, METAP2
Positive Sample:
SH-SY5Y, HL-60, Mouse liver
Cellular Localization:
Cytoplasm.
Calculated MW:
53kDa
Observed MW:
60kDa
The protein encoded by this gene is a member of the methionyl aminopeptidase family. The encoded protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent proteins. Increased expression of this gene is associated with various forms of cancer, and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. Inhibitors of this gene have also been shown to be effective for the treatment of obesity. A pseudogene of this gene is located on chromosome 2. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
10988
RRID
AB_2766687
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using METAP2 Rabbit pAb (CAB5962) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
