The METTL7B Antibody (CAB7200) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables thiol S-methyltransferase activity. Predicted to be involved in methylation. RRID AB_2767749 Gene ID 196410 Swiss Prot Synonym ALDI; METTL7B
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
METTL7B Antibody
SKU:
CAB7200
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:1000
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ALDI, METTL7B
Positive Sample:
HT-29, Mouse liver, Rat liver
Cellular Localization:
Lipid Droplet.
Calculated MW:
28kDa
Observed MW:
28kDa
Enables thiol S-methyltransferase activity. Predicted to be involved in methylation. RRID AB_2767749 Gene ID 196410 Swiss Prot Synonym ALDI; METTL7B
Purification Method:
Affinity purification
Gene ID:
196410
RRID:
AB_2767749
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat liver, using METTL7B Rabbit pAb (CAB7200) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of various lysates using METTL7B Rabbit pAb (CAB7200) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of U-251MG cells using METTL7B Rabbit pAb (CAB7200) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
