The METTL7B Antibody (CAB7200) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.Mettl7B is a member of the methyltransferase family and is involved in the methylation of RNA molecules, contributing to the regulation of gene expression. Research has shown that dysregulation of Mettl7B can impact various cellular functions, making it a potential target for studying diseases such as cancer, neurological disorders, and developmental abnormalities.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
METTL7B Antibody
SKU:
CAB7200
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ALDI, METTL7B
Positive Sample:
HT-29, Mouse liver, Rat liver
Cellular Localization:
Lipid Droplet.
Calculated MW:
28kDa
Observed MW:
28kDa
Enables thiol S-methyltransferase activity. Predicted to be involved in methylation.
Purification Method
Affinity purification
Gene ID
196410
RRID
AB_2767749
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat liver, using METTL7B Rabbit pAb (CAB7200) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of various lysates using METTL7B Rabbit pAb (CAB7200) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of U-251MG cells using METTL7B Rabbit pAb (CAB7200) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
