The MGAT2 Antibody (CAB9134) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications.MGAT2 plays a crucial role in the processing and modification of glycoproteins, which are essential for various biological processes including cell signaling, protein folding, and immune responses. By targeting MGAT2 with this specific antibody, researchers can investigate the function and regulation of this enzyme in different cell types, providing insights into its role in diseases such as cancer, diabetes, and infectious diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MGAT2 Antibody
SKU:
CAB9134
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GNT2, CDG2A, CDGS2, GNT-II, GLCNACTII, MGAT2
Positive Sample:
K-562, Mouse kidney, Rat kidney
Cellular Localization:
Golgi Apparatus Membrane, Single-Pass Type Ii Membrane Protein.
Calculated MW:
52kDa
Observed MW:
60kDa
The product of this gene is a Golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. Mutations in this gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. The coding region of this gene is intronless. Transcript variants with a spliced 5' UTR may exist, but their biological validity has not been determined.
Purification Method
Affinity purification
Gene ID
4247
RRID
AB_2770355
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MGAT2 Rabbit pAb (CAB9134) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
