The MGAT3 Monoclonal Antibody (CAB24347) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated using cutting-edge technology, exhibits high specificity and sensitivity for detecting MGAT3 in human samples, making it ideal for various research applications such as Western blotting and immunofluorescence.MGAT3 plays a crucial role in the modification of glycoproteins, impacting processes like cell-cell interactions, cell signaling, and immune responses.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
MGAT3 Monoclonal Antibody
SKU:
CAB24347
Size:
20μL, 100μL
Reactivity:
Mouse
Clone Number:
ARC60778
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GNT3, GNT-III, MGAT3
Positive Sample:
Mouse kidney
Cellular Localization:
Golgi Apparatus Membrane, Single-Pass Type Ii Membrane Protein.
Calculated MW:
61kDa
Observed MW:
62kDa
There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. The enzyme encoded by this gene transfers a GlcNAc residue to the beta-linked mannose of the trimannosyl core of N-linked oligosaccharides and produces a bisecting GlcNAc. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Purification Method
Affinity purification
Gene ID
4248
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MGAT3 Rabbit mAb (CAB24347) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): mouse skeletal muscle. Exposure time: 45s.