The MIER1 Antibody (CAB18480) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a protein that was first identified in Xenopus laevis by its role in a mesoderm induction early response (MIER). The encoded protein functions as a transcriptional regulator. Alternatively spliced transcript variants encode multiple isoforms, some of which lack a C-terminal nuclear localization signal. RRID AB_2862247 Gene ID 57708 Swiss Prot Synonym ER1; MI-ER1; MIER1
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
MIER1 Antibody
SKU:
CAB18480
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
-
Conjugate:
-
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ER1, MI-ER1, MIER1
Positive Sample:
HeLa, A-549, Mouse brain
Cellular Localization:
Cytoplasm, Nucleoplasm, Nucleus.
Calculated MW:
58kDa
Observed MW:
58kDa
This gene encodes a protein that was first identified in Xenopus laevis by its role in a mesoderm induction early response (MIER). The encoded protein functions as a transcriptional regulator. Alternatively spliced transcript variants encode multiple isoforms, some of which lack a C-terminal nuclear localization signal. RRID AB_2862247 Gene ID 57708 Swiss Prot Synonym ER1; MI-ER1; MIER1
Purification Method:
Affinity purification
Gene ID:
57708
RRID:
AB_2862247
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MIER1 Rabbit pAb (CAB18480) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using MIER1 Rabbit pAb (CAB18480) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
