The MIOX Antibody (CAB14409) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables ferric iron binding activity and inositol oxygenase activity. Involved in inositol catabolic process. Predicted to be located in cytoplasm and inclusion body.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
MIOX Antibody
SKU:
CAB14409
Size:
100μL, 20μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISAIF-P
Recommended Dilution:
WB
1:500 - 1:2000
IF-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ALDRL6, MIOX
Positive Sample:
Mouse kidney, Rat kidney
Cellular Localization:
Cytoplasm.
Calculated MW:
33kDa
Observed MW:
33kDa
Enables ferric iron binding activity and inositol oxygenase activity. Involved in inositol catabolic process. Predicted to be located in cytoplasm and inclusion body.
Purification Method
Affinity purification
Gene ID
55586
RRID
AB_2761277
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MIOX Rabbit pAb (CAB14409) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 15s.
Immunofluorescence analysis of paraffin-embedded Rat kidney using MIOX Rabbit pAb (CAB14409) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
