Description
| Product Name: | Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit |
| Product Code: | MAES0240 |
| Sample Type: | Animal Tissue |
| Assay Time: | 15 min |
| Assay Type: | Enzyme Activity |
| Detection Instrument: | Microplate reader (340 nm) |
| Detection Method: | Colorimetric method |
| Sensitivity: | 15.58 U/L |
| Detection Range: | 15.58-224.2 U/L |
| Inter-assay CV: | 0.084 |
| Intra-assay CV: | 0.008 |
Mitochondrial complex I catalyzes the reaction of NADH with ubiquinone substrate to generate NAD+ and reduced ubiquinone. The specific inhibitor rotenone can inhibit the enzyme, and the total enzyme activity minus the enzyme activity after inhibitor inhibition can reflect its specific enzyme activity.
| Storage: | -20°C |
| Valid Period: | 12 months |
| Other Reagents Required: | Anhydrous ethanol (AR) |
| Other Instruments Required: | Centrifuge, 37℃ incubator |
