Description
| Product Name: | Mitochondrial Complex II Activity Assay Kit |
| Product Code: | MAES0223 |
| Sample Type: | Animal Tissue, Cell |
| Assay Time: | 15 min |
| Assay Type: | Enzyme Activity |
| Detection Instrument: | Microplate reader (590-610 nm, optimum wavelength: 600 nm) |
| Detection Method: | Colorimetric method |
| Sensitivity: | 0.54 U/L |
| Detection Range: | 0.54-17.66 U/L |
| Inter-assay CV: | 0.05 |
| Intra-assay CV: | 0.04 |
Coenzyme Q, the catalytic product of mitochondrial complex II, can further reduce 2, 6-dichloroindoxol, which has a characteristic absorption peak at 600 nm. Therefore, the activity of mitochondrial complex II can be quantified by measure the change OD value at 600 nm.
| Storage: | 2-8°C |
| Valid Period: | 12 months |
| Other Reagents Required: | Normal saline (0.9% NaCl), PBS(0.01 M，pH 7.4) |
| Other Instruments Required: | Centrifuge |
