Description
| Product Name: | Mitochondrial Respiratory Chain ComplexⅤ(F0F1-ATPase /ATP Synthase) Activity Assay Kit |
| Product Code: | MAES0250 |
| Sample Type: | Animal Tissue, Cell |
| Assay Time: | 15 min |
| Assay Type: | Enzyme Activity |
| Synonyms: | ATP Synthase |
| Detection Instrument: | Microplate reader (330-350 nm, optimum wavelength: 340 nm) |
| Detection Method: | Colorimetric method |
| Sensitivity: | 12.13 U/L |
| Detection Range: | 12.13-74.21 U/L |
| Inter-assay CV: | 0.065 |
| Intra-assay CV: | 0.056 |
Mitochondrial complex V is also known as F0F1-ATP synthase. ATP is hydrolyzed by F0F1-ATP synthase to produce ADP, and ADP converts NADH into oxidized coenzyme I (NAD) after enzyme conversion reaction. Therefore, the activity of mitochondrial complex IV can be quantified by measure the change OD value at 340 nm.
| Storage: | -20°C |
| Valid Period: | 12 months |
| Other Instruments Required: | Centrifuge, 37℃ incubator |
