The MKP1 Polyclonal Antibody (CAB23261) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blotting.MKP1 is a key regulator of MAP kinase signaling pathways, playing a critical role in the control of cellular responses to extracellular stimuli. Dysregulation of MKP1 has been implicated in various diseases, making it a valuable target for research in cancer, inflammation, and other pathologies.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MKP1 Polyclonal Antibody
SKU:
CAB23261
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
erp, MKP1, mkp-1, 3CH134, Ptpn16
Positive Sample:
HeLa, HL-60, Mouse lung, Rat lung, Rat testis
Calculated MW:
39kDa
Observed MW:
40kDa
Enables mitogen-activated protein kinase binding activity and phosphoprotein phosphatase activity. Involved in negative regulation of MAPK cascade; negative regulation of cell population proliferation; and protein dephosphorylation. Predicted to be active in cytoplasm and nucleus. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; limb; and sensory organ. Human ortholog(s) of this gene implicated in breast cancer. Orthologous to human DUSP1 (dual specificity phosphatase 1).
Purification Method
Affinity purification
Gene ID
19252
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using MKP1 Rabbit pAb (CAB23261) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 2s.
Western blot analysis of various lysates, using MKP1 Rabbit pAb (CAB23261) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 2s.
