The MLANA Antibody (CAB6290) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific in recognizing MLANA in human samples and is validated for use in Western blot applications. By binding to the MLANA protein, this antibody enables accurate detection and analysis in various cell types, making it ideal for investigations in cancer research and immunotherapy.MLANA, also known as Melan-A, is a marker expressed by melanocytes and melanoma cells, playing a crucial role in melanocyte differentiation and melanoma progression.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MLANA Antibody
SKU:
CAB6290
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MART1, MART-1, Melan-A/MART-1
Positive Sample:
SK-MEL-28, OVCAR3
Cellular Localization:
Endoplasmic Reticulum Membrane, Golgi Apparatus, Melanosome, Single-Pass Type Iii Membrane Protein, Trans-Golgi Network Membrane.
Calculated MW:
13kDa
Observed MW:
19kDa
Located in endoplasmic reticulum membrane; melanosome; and trans-Golgi network.
Purification Method
Affinity purification
Gene ID
2315
RRID
AB_2766895
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Melan-A/MART-1 Rabbit pAb (CAB6290) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Immunofluorescence analysis of A375 cells using Melan-A/MART-1 Rabbit pAb (CAB6290) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
