The MLF1 Antibody (CAB8012) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly reactive with human samples and is validated for use in Western blot applications. By binding to the MLF1 protein, it enables researchers to detect and analyze this important molecule in various cell types, making it an ideal tool for studies in molecular biology and cancer research.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
MLF1 Antibody
SKU:
CAB8012
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MLF1
Positive Sample:
HeLa, BT-474, Mouse testis
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
31kDa
Observed MW:
35kDa
This gene encodes an oncoprotein which is thought to play a role in the phenotypic determination of hemopoetic cells. Translocations between this gene and nucleophosmin have been associated with myelodysplastic syndrome and acute myeloid leukemia. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
4291
RRID
AB_2770373
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MLF1 Rabbit pAb (CAB8012) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of A549 cells using MLF1 Rabbit pAb (CAB8012).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution.
